A physical map of Spodoptera littoralis B-type nuclear polyhedrosis virus genome. Brief report
Auteurs : Croizier (Guy), Croizier (Liliane) et Boukhoudmi-Amiri (K.)
Année de publication : 1989
Publication : Archives of Virology
Volume :
104
Pagination : 145-151
Résumé :
A physical map for the genome of Spodoptera littoralis (Egyptian cottonworm) nuclear polyhedrosis virus of B-type (S 1MNPV-B) [Cherry CL, Summers MD (1985) J Invertebr Pathol 46: 289–295] was prepared using the restriction endonucleases SmaI, SfiI, NotI, ApaI, KpnI, BstEII, PstI, SacI, and HindIII. The size of the genome was estimated to 136 kbp for the Morocco isolate (S 1MNPV-M2) and to 135 kbp for the Lyon isolate (S 1MNPV-L4).HindIII-K was used as the zero-point of the map because it hybridized toEcoRI-I andBamHI-F fragment of Autographa californica (AcMNPV) DNA, and the direction of transcription of theS 1MNPV polyhedrin gene was choosen left to right. Hybridization of sixAcMNPV cloned DNA fragments withS 1MNPV-B genome shows thatS 1MNPV-B and AcMNPV genomic structures are not aligned.